The Effect of follicular fluid on in Vitro Maturation of Mouse Immature Oocytes

Objective:In vitro maturation is a highly effective method for achieving mature  oocytes to treat infertility based on in vitro methods (IVF-ICSI). This technique is effective for treating women who suffer from polycystic ovary syndrome (PCO) and cancer. MATERIALS AND METHODS: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units of PMSG. The collected oocytes were divided into two groups: 1.The control group including the base medium 2. The experimental group including base medium supplemented with 25% human follicular fluid.100 oocytes (50 GV oocytes and 50 MI oocytes) were considered for each group. The oocytes of each group were cultured in incubator with 5% CO2 at 37 ºC for 24 hours. Results:Although the resumption of meiosis in the experimental group was more than the control group (44 and 33%, respectively), the difference was not statistically significant (PV>0.05). Moreover, among immature oocytes, the development of GV oocytes to mature oocyte was more than MI oocytes. 50%, 38% of GV oocytes and 28%, 38% of MI oocytes, respectively in the experimental and control groups reached to mature oocyte Conclusion: Results of the present study demonstrated that a higher percentage of immature oocytes in the experimental group matured in to MII oocytes. It seems that adding follicular fluid to the culture media has positive effects on oocyte maturation.  Moreover, GV oocytes are more capable to reach in MII stage compared with MI oocytes in vitro.